Recombinant Human CD33 / Siglec-3 Protein, with Fc Tag, expressed from HEK293

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PRODUCT

  • SKU

    YCP2045-100ug

  • Size

    100ug

  • Price

    $435

1
  • SKU

    YCP2045-1mg

  • Size

    1mg

  • Price

    $2,400

1
Human Siglec-3 is fused with a human IgG1 Fc tag at the C-terminus, and has a calculated MW of 52.9 kDa. The reducing (R) protein migrates at 64-85 kDa in SDS-PAGE due to glycosylation. Protein sequence: Asp 18 - His 259 of UniProt Accession Number P20138
gene name CD33
alias p67, SIGLEC3, SIGLEC-3, CD33
host species Human
production host HEK293
tags Fc Tag
purity >95% as determined by SDS-PAGE
endotoxin The protein was purified from endotoxin-free mammalian cell culture under strict endotoixn-free conditions. No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation Protein in [50 mM tris, 100 mM glycine, pH7.5] was 0.22 ?m filtered before lyophilized. Usually protectant (mannitol or trehalose) was added before lyophilization.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
concentration Lyophilized Powder
shipping Shipped at ambient conditions.
storage Lyophilized form is stable for 2 weeks at room temperature or for 12 months at 4-8°C. Keep at -20°C or lower for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

SDS-PAGE Analysis


PRODUCT

  • SKU

    YCP2045-100ug

  • Size

    100ug

  • Price

    $435

1
  • SKU

    YCP2045-1mg

  • Size

    1mg

  • Price

    $2,400

1