Recombinant Human TNFRSF18 / GITR Protein, with Mouse Fc Tag, expressed from HEK293

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PRODUCT

  • SKU

    YCP8286-100ug

  • Size

    100ug

  • Price

    $450

1
  • SKU

    YCP8286-1mg

  • Size

    1mg

  • Price

    $2,970

1
This recombinant protein contains AA Gln 26 - Glu 161 of accession number NP_004186.1. Its calculated molecular weight is 41.4 kDa. In SDS-PAGE, the protein migrates at 46-49 kDa and 50-55 kDa under reducing (R) condition due to glycosylation.
gene name TNFRSF18
alias AITR, GITR, TNFRSF18, CD357
host species Human
production host HEK293
tags Mouse Fc
purity >95% as determined by SDS-PAGE.
endotoxin The protein was purified from endotoxin-free mammalian cell culture under strict endotoixn-free conditions. No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 0.1 EU/ug).
formulation Protein in production buffer (Tris with Glycine, Arginine and NaCl, pH7.5) was 0.22 um filtered before lyophilization. Usually mannitol or trehalose was added as protectant from lyophilization.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
concentration Lyophilized Powder.
shipping Shipped at ambient conditions.
storage Lyophilized form is stable for 2 weeks at room temperature or for 12 months at 4-8°C. Keep at -20°C or lower for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

SDS-PAGE analysis of TNFRSF18 protein

ELISA: Plate-coupled Human GITR Ligand at 5 ug/mL (100 uL/well) binds to Human GITR with a linear range of 0.039-0.313 ug/mL.


PRODUCT

  • SKU

    YCP8286-100ug

  • Size

    100ug

  • Price

    $450

1
  • SKU

    YCP8286-1mg

  • Size

    1mg

  • Price

    $2,970

1