Recombinant Rat TIGIT Protein, with Fc Tag, expressed from HEK293

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PRODUCT

  • SKU

    YCP8573-50ug

  • Size

    50ug

  • Price

    $525

1
  • SKU

    YCP8573-1mg

  • Size

    1mg

  • Price

    $2,970

1
This recombinant protein contains AA Ala 17 - Ala 138 of accession number D3ZTQ2. Its calculated molecular weight is 40 kDa. As a result of glycosylation, In SDS-PAGE, the protein migrates at 48-55 kDa under reducing (R) condition, and 85-110 kDa under non-reducing (NR) condition.
gene name TIGIT
alias TIGIT, VSIG9, VSTM3
host species Rat
production host HEK293
tags Fc Tag
purity >95% as determined by SDS-PAGE.
endotoxin The protein was purified from endotoxin-free mammalian cell culture under strict endotoixn-free conditions. No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation Protein in production buffer (Tris with Glycine, Arginine and NaCl, pH7.5) was 0.22 um filtered before lyophilization. Usually mannitol or trehalose was added as protectant from lyophilization.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
concentration Lyophilized Powder.
shipping Shipped at ambient conditions.
storage Lyophilized form is stable for 2 weeks at room temperature or for 12 months at 4-8°C. Keep at -20°C or lower for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

SDS-PAGE analysis of TIGIT protein

ELISA: Plate-coupled Human Nectin-2 at 5 ug/mL (100 uL/well) binds to Rat TIGIT with a linear range of 0.039-5 ug/mL.


PRODUCT

  • SKU

    YCP8573-50ug

  • Size

    50ug

  • Price

    $525

1
  • SKU

    YCP8573-1mg

  • Size

    1mg

  • Price

    $2,970

1