Recombinant Cynomolgus / Rhesus macaque CD80 / B7-1 Protein, with His Tag, expressed from HEK293

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PRODUCT

  • SKU

    YCP5107-100ug

  • Size

    100ug

  • Price

    $390

1
  • SKU

    YCP5107-1mg

  • Size

    1mg

  • Price

    $2,640

1
This recombinant protein contains AA Val 35 - Asn 242 of accession number G7NXN7. Its calculated molecular weight is 25.8 kDa. In SDS-PAGE, the protein migrates at 43-55 kDa under reducing (R) condition due to different glycosylation.
gene name CD80
alias CD80, B7, B7-1, B7.1, BB1, CD28LG, CD28LG1, LAB7
host species Cynomolgus / Rhesus macaque
production host HEK293
tags His Tag
purity >95% as determined by SDS-PAGE.
endotoxin The protein was purified from endotoxin-free mammalian cell culture under strict endotoixn-free conditions. No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation Protein in production buffer (PBS, pH7.4) was 0.22 um filtered before lyophilization. Usually mannitol or trehalose was added as protectant from lyophilization.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
concentration Lyophilized Powder.
shipping Shipped at ambient conditions.
storage Lyophilized form is stable for 2 weeks at room temperature or for 12 months at 4-8°C. Keep at -20°C or lower for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

SDS-PAGE analysis of CD80 protein

ELISA: Plate-coupled Human CTLA-4 at 5 ug/mL (100 uL/well) binds to Cynomolgus / Rhesus macaque B7-1 with a linear range of 10-156 ng/mL.

ELISA: Plate-coupled Cynomolgus / Rhesus macaque CTLA-4 at 5 ug/mL (100 uL/well) binds to Cynomolgus / Rhesus macaque B7-1 with a linear range of 10-156 ng/mL.

SEC-HPLC analysis shows protein purity > 90%


PRODUCT

  • SKU

    YCP5107-100ug

  • Size

    100ug

  • Price

    $390

1
  • SKU

    YCP5107-1mg

  • Size

    1mg

  • Price

    $2,640

1