Recombinant Human CD80 / B7-1 Protein, with Mouse Fc Tag, expressed from HEK293

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PRODUCT

  • SKU

    YCP8027-100ug

  • Size

    100ug

  • Price

    $390

1
  • SKU

    YCP8027-1mg

  • Size

    1mg

  • Price

    $2,640

1
This recombinant protein contains AA Val 35 - Asn 242 of accession number NP 005182.1. Its calculated molecular weight is 50.9 kDa. In SDS-PAGE, the protein migrates at 65-100 kDa under reducing (R) condition due to glycosylation.
gene name CD80
alias CD80, B7, B7-1, B7.1, BB1, CD28LG, CD28LG1, LAB7
host species Human
production host HEK293
tags Mouse Fc
purity >95% as determined by SDS-PAGE.
endotoxin The protein was purified from endotoxin-free mammalian cell culture under strict endotoixn-free conditions. No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 0.1 EU/ug).
formulation Protein in production buffer (Tris with Glycine, Arginine and NaCl, pH7.5) was 0.22 um filtered before lyophilization. Usually mannitol or trehalose was added as protectant from lyophilization.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
concentration Lyophilized Powder.
shipping Shipped at ambient conditions.
storage Lyophilized form is stable for 2 weeks at room temperature or for 12 months at 4-8°C. Keep at -20°C or lower for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

SDS-PAGE analysis of CD80 protein

ELISA: Plate-coupled Human B7-1 at 5 ug/mL (100 uL/well) binds to Human PD-L1 with a linear range of 0.01-0.313 ug/mL.

ELISA: Plate-coupled Human CTLA-4 at 1 ug/mL (100 uL/well) binds to Human B7-1 with a linear range of 2-31 ng/mL.


PRODUCT

  • SKU

    YCP8027-100ug

  • Size

    100ug

  • Price

    $390

1
  • SKU

    YCP8027-1mg

  • Size

    1mg

  • Price

    $2,640

1