Recombinant Human CD3E & CD3D Heterodimer Protein (CD3E-Fc-His, CD3D-Fc-Flag), expressed from HEK293

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PRODUCT

  • SKU

    YCP5197-100ug

  • Size

    100ug

  • Price

    $435

1
  • SKU

    YCP5197-1mg

  • Size

    1mg

  • Price

    $2,400

1
Human CD3 epsilon & CD3 delta Protein is produced by co-expression of CD3E and CD3D, has a calculated MW of 39.2 kDa (CD3E), 37.5 kDa (CD3D) . Subunit CD3E is fused with his-tagged human IgG1 Fc fragment at the C-terminus and subunit CD3D is fused with flag-tagged human IgG1 Fc fragment at the C-terminus. DTT-reduced heterodimer protein migrates at 40-55 kDa due to glycosylation. Protein sequence: Asp 23 - Asp 126 (CD3E) & Phe 22 - Ala 105 (CD3D) of UniProt Accession Number P07766 (CD3E) & P04234 (CD3D)
gene name CD3E & CD3D
alias CD3, CD3E, CD3G
host species Human
production host HEK293
tags His Tag & Fc Tag & Flag Tag
purity >95% as determined by SDS-PAGE
endotoxin The protein was purified from endotoxin-free mammalian cell culture under strict endotoixn-free conditions. No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation Protein in [PBS, pH7.4] was 0.22 ?m filtered before lyophilized. Usually protectant (mannitol or trehalose) was added before lyophilization.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
concentration Lyophilized Powder
shipping Shipped at ambient conditions.
storage Lyophilized form is stable for 2 weeks at room temperature or for 12 months at 4-8°C. Keep at -20°C or lower for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

PRODUCT

  • SKU

    YCP5197-100ug

  • Size

    100ug

  • Price

    $435

1
  • SKU

    YCP5197-1mg

  • Size

    1mg

  • Price

    $2,400

1