Recombinant Human CD3E & CD3G Heterodimer Protein (CD3E-Fc-His, CD3G-Fc-Flag), expressed from HEK293

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PRODUCT

  • SKU

    YCP6021-50ug

  • Size

    50ug

  • Price

    $472.5

1
  • SKU

    YCP6021-1mg

  • Size

    1mg

  • Price

    $4,200

1
The heterodimer Human CD3 epsilon & CD3 gamma is produced by co-expression of CD3E and CD3G, has a calculated MW of 39.2 kDa (CD3E) and 38.6 kDa (CD3D). Subunit CD3E is fused with a human IgG1 Fc tag at the C-terminus, followed by 6xHis Tag, and subunit CD3G is fused with a human IgG1 Fc tag at the C-terminus, followed by a Flag tag. The reducing (R) heterodimer protein migrates at 40-50 kDa due to glycosylation. Protein sequence: Asp 23 - Asp 126 (CD3E) & Gln 23 - Ser 116 (CD3G) of UniProt Accession Number P07766 (CD3E) & NP_000064.1 (CD3G)
gene name CD3E & CD3G
alias CD3, CD3E, CD3G
host species Human
production host HEK293
tags His Tag & Fc Tag & Flag Tag
purity >95% as determined by SDS-PAGE.
endotoxin The protein was purified from endotoxin-free mammalian cell culture under strict endotoixn-free conditions. No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation Protein in [PBS, pH7.4] was 0.22 ?m filtered before lyophilized. Usually protectant (mannitol or trehalose) was added before lyophilization.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
concentration Lyophilized Powder
shipping Shipped at ambient conditions.
storage Lyophilized form is stable for 2 weeks at room temperature or for 12 months at 4-8°C. Keep at -20°C or lower for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

PRODUCT

  • SKU

    YCP6021-50ug

  • Size

    50ug

  • Price

    $472.5

1
  • SKU

    YCP6021-1mg

  • Size

    1mg

  • Price

    $4,200

1