Recombinant Cynomolgus CD3E / CD3 epsilon Protein, with His Tag & Fc Tag, expressed from HEK293

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PRODUCT

  • SKU

    YCP5202-100ug

  • Size

    100ug

  • Price

    $525

1
  • SKU

    YCP5202-1mg

  • Size

    1mg

  • Price

    $2,970

1
This recombinant protein contains AA Gln 22 - Asp 117 of accession number Q95LI5. This protein carries a human IgG1 Fc tag at the C-terminus, followed by a polyhistidine tag.Its calculated molecular weight is 38.3 kDa. In SDS-PAGE, the protein migrates at 43-50 kDa under reducing (R) condition due to glycosylation.
gene name CD3E
alias FLJ18683, T3E, TCRE, CD3E, CD3-epsilon
host species Cynomolgus
production host HEK293
tags His Tag & Fc Tag
purity >95% as determined by SDS-PAGE.
endotoxin The protein was purified from endotoxin-free mammalian cell culture under strict endotoixn-free conditions. No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation Protein in production buffer (Tris with Glycine, Arginine and NaCl, pH7.5) was 0.22 um filtered before lyophilization. Usually mannitol or trehalose was added as protectant from lyophilization.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
concentration Lyophilized Powder.
shipping Shipped at ambient conditions.
storage Lyophilized form is stable for 2 weeks at room temperature or for 12 months at 4-8°C. Keep at -20°C or lower for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

SDS-PAGE analysis of CD3E protein

ELISA: Plate-coupled SP34-2 at 2 ug/mL (100 uL/well) binds to Cynomolgus CD3 epsilon, Fc,His Tag with a linear range of 0.2-3 ng/mL.


PRODUCT

  • SKU

    YCP5202-100ug

  • Size

    100ug

  • Price

    $525

1
  • SKU

    YCP5202-1mg

  • Size

    1mg

  • Price

    $2,970

1