Recombinant Human CD3E / CD3 epsilon Protein, with His Tag & Fc Tag, expressed from HEK293

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PRODUCT

  • SKU

    YCP5204-100ug

  • Size

    100ug

  • Price

    $435

1
  • SKU

    YCP5204-1mg

  • Size

    1mg

  • Price

    $2,805

1
This recombinant protein contains AA Asp 23 - Asp 126 of accession number NP_000724.1. This protein carries a human IgG1 Fc tag at the C-terminus, followed by a polyhistidine tag.Its calculated molecular weight is 39.2 kDa. In SDS-PAGE, the protein migrates at 45-55 kDa under reducing (R) condition due to glycosylation.
gene name CD3E
alias FLJ18683, T3E, TCRE, CD3E, CD3-epsilon
host species Human
production host HEK293
tags His Tag & Fc Tag
purity >95% as determined by SDS-PAGE.
endotoxin The protein was purified from endotoxin-free mammalian cell culture under strict endotoixn-free conditions. No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation Protein in production buffer (PBS, pH7.4) was 0.22 um filtered before lyophilization. Usually mannitol or trehalose was added as protectant from lyophilization.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
concentration Lyophilized Powder.
shipping Shipped at ambient conditions.
storage Lyophilized form is stable for 2 weeks at room temperature or for 12 months at 4-8°C. Keep at -20°C or lower for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

SDS-PAGE analysis of CD3E protein

ELISA: Plate-coupled Anti Human CD3 MAb, mouse IgG1 (Clone # SP34-2) at 2 ug/mL (100 uL/well) binds to Human CD3 epsilon, Fc,His Tag with a linear range of 0.1-3 ng/mL.


PRODUCT

  • SKU

    YCP5204-100ug

  • Size

    100ug

  • Price

    $435

1
  • SKU

    YCP5204-1mg

  • Size

    1mg

  • Price

    $2,805

1