Recombinant Rat PCSK9 Protein, with His Tag, expressed from HEK293

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PRODUCT

  • SKU

    YCP5719-50ug

  • Size

    50ug

  • Price

    $390

1
  • SKU

    YCP5719-1mg

  • Size

    1mg

  • Price

    $2,970

1
This recombinant protein contains AA Gln 31 - Gln 691 of accession number NP_954862.2. Its calculated molecular weight is 73.2 kDa. In SDS-PAGE, the protein migrates at 20 kDa and 63-66 kDa under reducing (R) condition due to glycosylation.
gene name PCSK9
alias PCSK9, FH3, HCHOLA3, LDLCQ1, NARC1, PC9
host species Rat
production host HEK293
tags His Tag
purity >95% as determined by reduced SDS-PAGE.
endotoxin The protein was purified from endotoxin-free mammalian cell culture under strict endotoixn-free conditions. No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation Protein in production buffer (50 mM Tris, 150 mM NaCl, pH7.5) was 0.22 um filtered before lyophilization. Usually mannitol or trehalose was added as protectant from lyophilization.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
concentration Lyophilized Powder.
shipping Shipped at ambient conditions.
storage Lyophilized form is stable for 2 weeks at room temperature or for 12 months at 4-8°C. Keep at -20°C or lower for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

SDS-PAGE analysis of PCSK9 protein

ELISA: Plate-coupled Human LDL R, Strep Tag at 2 ug/mL (100 uL/well) binds to Rat PCSK9 with a linear range of 8-63 ng/mL.


PRODUCT

  • SKU

    YCP5719-50ug

  • Size

    50ug

  • Price

    $390

1
  • SKU

    YCP5719-1mg

  • Size

    1mg

  • Price

    $2,970

1