Recombinant Human PCSK9 (R194A) (31-692) Protein, with His Tag, expressed from HEK293

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PRODUCT

  • SKU

    YCP5721-50ug

  • Size

    50ug

  • Price

    $390

1
  • SKU

    YCP5721-1mg

  • Size

    1mg

  • Price

    $2,970

1
This recombinant protein contains AA Gln 31 - Gln 692 of accession number AAI166619.1, with R194A mutation. It has a calculated MW of 71.8 kDa. DTT-reduced Protein migrates at 17 kDa and 66 kDa in SDS-PAGE due to glycosylation and proteolytic digestion. The R194A mutation results in lower affinity of PCSK9 for LDLR.
gene name PCSK9
alias PCSK9, FH3, HCHOLA3, LDLCQ1, NARC1, PC9
host species Human
production host HEK293
tags His Tag
purity >95% as determined by SDS-PAGE.
endotoxin The protein was purified from endotoxin-free mammalian cell culture under strict endotoixn-free conditions. No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation Protein in production buffer (PBS, pH7.4) was 0.22 um filtered before lyophilization. Usually mannitol or trehalose was added as protectant from lyophilization.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
concentration Lyophilized Powder.
shipping Shipped at ambient conditions.
storage Lyophilized form is stable for 2 weeks at room temperature or for 12 months at 4-8°C. Keep at -20°C or lower for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

SDS-PAGE analysis of PCSK9 protein

ELISA: Plate-coupled Human LDL R at 10 ug/mL (100 uL/well) binds to Human PCSK9 (R194A) with a linear range of 0.1-2 ug/mL.


PRODUCT

  • SKU

    YCP5721-50ug

  • Size

    50ug

  • Price

    $390

1
  • SKU

    YCP5721-1mg

  • Size

    1mg

  • Price

    $2,970

1