Recombinant Cynomolgus PDCD1 / PD-1 Protein, with Fc Tag, expressed from HEK293

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PRODUCT

  • SKU

    YCP5727-100ug

  • Size

    100ug

  • Price

    $450

1
  • SKU

    YCP5727-1mg

  • Size

    1mg

  • Price

    $2,700

1
This recombinant protein contains AA Leu 25 - Gln 167 of accession number B0LAJ3. Its calculated molecular weight is 42.6 kDa. In SDS-PAGE, the protein migrates at 53-60 kDa under reducing (R) condition due to different glycosylation.
gene name PDCD1
alias PDCD1, PD1, CD279, SLEB2, PD-1
host species Cynomolgus
production host HEK293
tags Fc Tag
purity >90% as determined by SDS-PAGE.
endotoxin The protein was purified from endotoxin-free mammalian cell culture under strict endotoixn-free conditions. No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation Protein in production buffer (50 mM Tris, 100 mM Glycine, pH7.5) was 0.22 um filtered before lyophilization. Usually mannitol or trehalose was added as protectant from lyophilization.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
concentration Lyophilized Powder.
shipping Shipped at ambient conditions.
storage Lyophilized form is stable for 2 weeks at room temperature or for 12 months at 4-8°C. Keep at -20°C or lower for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

SDS-PAGE analysis of PDCD1 protein

ELISA: Plate-coupled Cynomolgus PD-1 at 2 ug/mL (100 uL/well) binds to Nivolumab with a linear range of 0.1-3 ng/mL.

ELISA: Plate-coupled Human PD-L1, Mouse Fc Tag at 5 ug/mL (100 uL/well) binds to Cynomolgus PD-1 with a linear range of 3-33 ng/mL.


PRODUCT

  • SKU

    YCP5727-100ug

  • Size

    100ug

  • Price

    $450

1
  • SKU

    YCP5727-1mg

  • Size

    1mg

  • Price

    $2,700

1