Recombinant Human PDCD1 / PD-1 Protein, with His Tag, expressed from HEK293, Endotoxin-free

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PRODUCT

  • SKU

    YCP8466-1mg

  • Size

    1mg

  • Price

    $3,600

1
This recombinant protein contains AA Leu 25 - Gln 167 of accession number NP_005009.2. This protein carries a polyhistidine tag at the C-terminus, and has a calculated MW of 16.8 kDa. The N-terminus Sequence Analysis is Leu 25. The reducing (R) protein migrates as 31-44 kDa in SDS-PAGE due to glycosylation.
gene name PDCD1
alias PDCD1, PD1, CD279, SLEB2, PD-1
host species Human
production host HEK293
tags His Tag
purity >95% as determined by SDS-PAGE.
endotoxin The protein was purified from endotoxin-free mammalian cell culture under strict endotoixn-free conditions. No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 0.01 EU/ug).
formulation Protein in production buffer (PBS, pH7.4) was 0.22 um filtered before lyophilization. Usually mannitol or trehalose was added as protectant from lyophilization.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
concentration Lyophilized Powder.
shipping Shipped at ambient conditions.
storage Lyophilized form is stable for 2 weeks at room temperature or for 12 months at 4-8°C. Keep at -20°C or lower for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

SDS-PAGE analysis of PDCD1 protein

ELISA: Plate-coupled Human PD-1 at 2 ug/mL (100 uL/well) binds to Human PD-L2, Mouse IgG1 Fc Tag with a linear range of 10-156 ng/mL.

SEC-HPLC analysis shows protein purity > 90%


PRODUCT

  • SKU

    YCP8466-1mg

  • Size

    1mg

  • Price

    $3,600

1