Recombinant Human Complement Component C5a (679-751) Protein, with Fc Tag, expressed from HEK293

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PRODUCT

  • SKU

    YCP8084-100ug

  • Size

    100ug

  • Price

    $525

1
  • SKU

    YCP8084-1mg

  • Size

    1mg

  • Price

    $3,465

1
This recombinant protein contains AA Leu 679 - Arg 751 of accession number XP_015292262.1. Its calculated molecular weight is 34.6 kDa. In SDS-PAGE, the protein migrates at 38-40 kDa and 41-44 kDa under reducing (R) condition due to glycosylation.
gene name C5
alias C5a, Complement Component 5a
host species Human
production host HEK293
tags Fc Tag
purity >90% as determined by SDS-PAGE.
endotoxin The protein was purified from endotoxin-free mammalian cell culture under strict endotoixn-free conditions. No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation Protein in production buffer (Tris with Glycine, Arginine and NaCl, pH7.5) was 0.22 um filtered before lyophilization. Usually mannitol or trehalose was added as protectant from lyophilization.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
concentration Lyophilized Powder.
shipping Shipped at ambient conditions.
storage Lyophilized form is stable for 2 weeks at room temperature or for 12 months at 4-8°C. Keep at -20°C or lower for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

SDS-PAGE analysis of C5 protein

ELISA: Plate-coupled Human C5a at 2 ug/mL (100 uL/well) binds to Anti-C5a (Human IgG1) with a linear range of 2-16 ng/mL.


PRODUCT

  • SKU

    YCP8084-100ug

  • Size

    100ug

  • Price

    $525

1
  • SKU

    YCP8084-1mg

  • Size

    1mg

  • Price

    $3,465

1