Recombinant Human Complement C5 (19-1676, R885H) Protein, with His Tag, expressed from HEK293

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PRODUCT

  • SKU

    YCP8201-100ug

  • Size

    100ug

  • Price

    $525

1
  • SKU

    YCP8201-1mg

  • Size

    1mg

  • Price

    $3,465

1
This recombinant protein contains AA Gln 19 - Cys 1676 of accession number NP_001726.2, with R885H mutation. The mature form of Complement C5 is a disulfide-linked heterodimer composed of proteolytically cleaved α and β chain. Each α and β chain has a calculated MW of 73.3 kDa (β chain) and 114.7 kDa (α chain). In SDS-PAGE, the protein migrates at 76 kDa (β chain) and 116 kDa (α chain) under reducing (R) condition due to glycosylation.
gene name C5
alias Complement C5, C5, CPAMD4
host species Human
production host HEK293
tags His Tag
purity >90% as determined by SDS-PAGE.
endotoxin The protein was purified from endotoxin-free mammalian cell culture under strict endotoixn-free conditions. No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation Protein in production buffer (PBS, pH7.4) was 0.22 um filtered before lyophilization. Usually mannitol or trehalose was added as protectant from lyophilization.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
concentration Lyophilized Powder.
shipping Shipped at ambient conditions.
storage Lyophilized form is stable for 2 weeks at room temperature or for 12 months at 4-8°C. Keep at -20°C or lower for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

SDS-PAGE analysis of C5 protein

ELISA: Plate-coupled Human Complement-C5 (R885H) at 2 ug/mL (100 uL/well) binds to Anti-C5a (Human IgG1) with a linear range of 0.2-6 ng/mL.


PRODUCT

  • SKU

    YCP8201-100ug

  • Size

    100ug

  • Price

    $525

1
  • SKU

    YCP8201-1mg

  • Size

    1mg

  • Price

    $3,465

1