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YCP8116-1mg
1mg
$4,500
| gene name | CD47 |
| alias | CD47, MER6, IAP, OA3 |
| host species | Human |
| production host | HEK293 |
| tags | His Tag |
| purity | >95% as determined by SDS-PAGE. |
| endotoxin | The protein was purified from endotoxin-free mammalian cell culture under strict endotoixn-free conditions. No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 0.01 EU/ug). |
| formulation | Protein in production buffer (PBS, pH7.4) was 0.22 um filtered before lyophilization. Usually mannitol or trehalose was added as protectant from lyophilization. |
| reconstitution | Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage. |
| concentration | Lyophilized Powder. |
| shipping | Shipped at ambient conditions. |
| storage | Lyophilized form is stable for 2 weeks at room temperature or for 12 months at 4-8°C. Keep at -20°C or lower for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months. |
SDS-PAGE analysis of CD47 protein
ELISA: Plate-coupled Anti-CD47 MAb, Human IgG4 (clone 5F9) at 2 ug/mL (100 uL/well) binds to Human CD47 with a linear range of 0.4-3 ng/mL.
ELISA: Plate-coupled Human SIRP alpha at 5 ug/mL (100 uL/well) binds to Human CD47 with a linear range of 2-13 ng/mL.
YCP8116-1mg
1mg
$4,500