Recombinant Human CD47 Protein, with His Tag & Avi Tag, expressed from HEK293

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PRODUCT

  • SKU

    YCP8123-50ug

  • Size

    50ug

  • Price

    $390

1
  • SKU

    YCP8123-1mg

  • Size

    1mg

  • Price

    $3,300

1
This recombinant protein contains AA Gln 19 - Pro 139 of accession number NP_942088. This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.Its calculated molecular weight is 16.9 kDa. In SDS-PAGE, the protein migrates at 33-44 kDa under reducing (R) condition due to glycosylation.
gene name CD47
alias CD47, MER6, IAP, OA3
host species Human
production host HEK293
tags His Tag & Avi Tag
purity >95% as determined by SDS-PAGE.
endotoxin The protein was purified from endotoxin-free mammalian cell culture under strict endotoixn-free conditions. No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation Protein in production buffer (10 mM Bicine, pH8.3) was 0.22 um filtered before lyophilization. Usually mannitol or trehalose was added as protectant from lyophilization.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
concentration Lyophilized Powder.
shipping Shipped at ambient conditions.
storage Lyophilized form is stable for 2 weeks at room temperature or for 12 months at 4-8°C. Keep at -20°C or lower for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

SDS-PAGE analysis of CD47 protein

ELISA: Plate-coupled Anti-CD47 MAb, Human IgG4 at 2ug/mL (100 uL/well) binds to Unconjugated Human CD47 with a linear range of 0.05-2.05 ng/mL.

ELISA: Plate-coupled Human SIRP alpha at 2ug/mL (100 uL/well) binds to Unconjugated Human CD47 with a linear range of 2-12.8 ng/mL.


PRODUCT

  • SKU

    YCP8123-50ug

  • Size

    50ug

  • Price

    $390

1
  • SKU

    YCP8123-1mg

  • Size

    1mg

  • Price

    $3,300

1