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YCP1997-100ug
100ug
$495
YCP1997-1mg
1mg
$3,300
gene name | CD274 |
alias | PD-L1, CD274, B7-H1, PDCD1L1, PDCD1LG1, PDL1 |
host species | Human |
production host | HEK293 |
tags | His Tag |
purity | >98% as determined by SDS-PAGE. |
endotoxin | The protein was purified from endotoxin-free mammalian cell culture under strict endotoixn-free conditions. No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug). |
formulation | Protein in production buffer (PBS, pH7.4) was 0.22 um filtered before lyophilization. Usually mannitol or trehalose was added as protectant from lyophilization. |
reconstitution | Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage. |
concentration | Lyophilized Powder. |
shipping | Shipped at ambient conditions. |
storage | Lyophilized form is stable for 2 weeks at room temperature or for 12 months at 4-8°C. Keep at -20°C or lower for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months. |
SDS-PAGE analysis of CD274 protein
ELISA: Plate-coupled Human PD-L1 (HPLC-verified) at series of concentration on MonRabTM Anti-His Tag (C-term) Antibody precoated (0.1 ug/well) plate, binds to Human PD-1 at 5 ug
ELISA: Plate-coupled Human PD-L1 (HPLC-verified) at 1 ug/mL (100 uL/well) binds to Anti-Human PD-L1 MAb (Human IgG1) with a linear range of 0.1-3 ng/mL.
SEC-HPLC analysis shows protein purity > 90%
YCP1997-100ug
100ug
$495
YCP1997-1mg
1mg
$3,300