Recombinant Human CD274 / PD-L1 Protein, with Strep Tag, expressed from HEK293

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PRODUCT

  • SKU

    YCP5733-25ug

  • Size

    25ug

  • Price

    $390

1
  • SKU

    YCP5733-1mg

  • Size

    1mg

  • Price

    $3,450

1
This recombinant protein contains AA Phe 19 - Arg 238 of accession number NP_054862.1 . Its calculated molecular weight is 28.2 kDa. In SDS-PAGE, the protein migrates at 35-42 kDa under reducing (R) condition due to glycosylation.
gene name CD274
alias PD-L1, CD274, B7-H1, PDCD1L1, PDCD1LG1, PDL1
host species Human
production host HEK293
tags Strep Tag
purity >95% as determined by SDS-PAGE.
endotoxin The protein was purified from endotoxin-free mammalian cell culture under strict endotoixn-free conditions. No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation Protein in production buffer (PBS, pH7.4) was 0.22 um filtered before lyophilization. Usually mannitol or trehalose was added as protectant from lyophilization.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
concentration Lyophilized Powder.
shipping Shipped at ambient conditions.
storage Lyophilized form is stable for 2 weeks at room temperature or for 12 months at 4-8°C. Keep at -20°C or lower for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

SDS-PAGE analysis of CD274 protein

ELISA: Plate-coupled Human PD-L1, Strep Tag at 5 ug/mL ( 100 uL/well ) binds to Biotinylated Human PD-1 & Fc tag with a linear range of 2-60 ng/mL.


PRODUCT

  • SKU

    YCP5733-25ug

  • Size

    25ug

  • Price

    $390

1
  • SKU

    YCP5733-1mg

  • Size

    1mg

  • Price

    $3,450

1