Recombinant Mouse CD274 / PD-L1 Protein, with Fc Tag, expressed from HEK293

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PRODUCT

  • SKU

    YCP5744-200ug

  • Size

    200ug

  • Price

    $405

1
  • SKU

    YCP5744-1mg

  • Size

    1mg

  • Price

    $1,800

1
This recombinant protein contains AA Phe 19 - Thr 238 of accession number NP_068693. Its calculated molecular weight is 51.4 kDa. In SDS-PAGE, the protein migrates at 70-95 kDa under reducing (R) condition due to glycosylation.
gene name CD274
alias PD-L1, CD274, B7-H1, PDCD1L1, PDCD1LG1, PDL1
host species Mouse
production host HEK293
tags Fc Tag
purity >95% as determined by SDS-PAGE.
endotoxin The protein was purified from endotoxin-free mammalian cell culture under strict endotoixn-free conditions. No endotoxin was detected in final prodcut using LAL method to the method's sensitivity level (< 1.0 EU/ug).
formulation Protein in production buffer (Tris with Glycine, Arginine and NaCl, pH7.5) was 0.22 um filtered before lyophilization. Usually mannitol or trehalose was added as protectant from lyophilization.
reconstitution Reconstitute lyophilized powder with water to a concentration of no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% of HSA be added as a carrier protein for long term storage.
concentration Lyophilized Powder.
shipping Shipped at ambient conditions.
storage Lyophilized form is stable for 2 weeks at room temperature or for 12 months at 4-8°C. Keep at -20°C or lower for long term storage. After reconstitution, store at 4°C for 2-7 days or at below -20°C for up to 3 months.

Images

SDS-PAGE analysis of CD274 protein

ELISA: Plate-coupled Mouse PD-L1 / B7-H1 Protein binds to Biotinylated Mouse PD-1 / PDCD1 . The linear range is 0.15-2.5 ug/mL.


PRODUCT

  • SKU

    YCP5744-200ug

  • Size

    200ug

  • Price

    $405

1
  • SKU

    YCP5744-1mg

  • Size

    1mg

  • Price

    $1,800

1